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ATG13 Monoclonal antibody, PBS Only

ATG13 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C3A7

Cat no : 66708-1-PBS

Print datasheet

Synonyms

KIAA0652, Autophagy-related protein 13, Autophagy related protein 13, 1C3A7

验证数据展示

  • WB analysis using 66708-1-Ig (same clone as 66708-1-PBS)

    WB analysis using 66708-1-Ig (same clone as 66708-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66708-1-Ig (ATG13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66708-1-Ig (same clone as 66708-1-PBS)

    IHC staining of mouse testis using 66708-1-Ig (same clone as 66708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

  • WB analysis of BGC-823 using 66708-1-Ig (same clone as 66708-1-PBS)

    WB analysis of BGC-823 using 66708-1-Ig (same clone as 66708-1-PBS)

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66708-1-Ig (same clone as 66708-1-PBS)

    WB analysis of NIH/3T3 using 66708-1-Ig (same clone as 66708-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66708-1-Ig (same clone as 66708-1-PBS)

    IF Staining of HeLa using 66708-1-Ig (same clone as 66708-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATG13 antibody (66708-1-Ig, Clone: 1C3A7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66708-1-Ig (same clone as 66708-1-PBS)

    FC experiment of HeLa using 66708-1-Ig (same clone as 66708-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATG13 (66708-1-Ig, Clone:1C3A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66708-1-PBS in a different storage buffer formulation.

产品信息

66708-1-PBS targets ATG13 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with pig, rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity pig, rat, mouse, human
Immunogen ATG13 fusion protein Ag12968 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name KIAA0652
Synonyms KIAA0652, Autophagy-related protein 13, Autophagy related protein 13, 1C3A7
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC001331
Gene Symbol ATG13
Gene ID (NCBI) 9776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75143
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)