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ATG16L2 Polyclonal antibody

ATG16L2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24322-1-AP

Print datasheet

Synonyms

APG16 like 2, ATG16L2, Autophagy related protein 16 2, WDR80

验证数据展示

  • IF Staining of MCF-7 using 24322-1-AP

    IF Staining of MCF-7 using 24322-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 24322-1-AP (ATG16L2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse spleen using 24322-1-AP

    WB analysis of mouse spleen using 24322-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 24322-1-AP (ATG16L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 24322-1-AP

    IP experiment of mouse testis using 24322-1-AP

    IP result of anti-ATG16L2 (IP:24322-1-AP, 3ug; Detection:24322-1-AP 1:700) with mouse testis tissue lysate 4800ug.

经过测试的应用

Positive WB detected inmouse spleen tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 24322-1-AP is ATG16L2 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen ATG16L2 fusion protein Ag19399 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG16 autophagy related 16-like 2 (S. cerevisiae)
Synonyms APG16 like 2, ATG16L2, Autophagy related protein 16 2, WDR80
Calculated Molecular Weight 619 aa, 69 kDa
Observed Molecular Weight 70 kDa, 30 kDa
GenBank Accession NumberBC137490
Gene Symbol ATG16L2
Gene ID (NCBI) 89849
RRIDAB_2879493
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NAA4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG16L2 also named as Autophagy related protein 16-2 is a 619 amino-acid protein, Which belongs to the WD repeat ATG16 family. ATG16L2 contains 7 WD repeats and localizes in the cytoplasm. There are three isoforms of ATG16L2 and the molecular weight of three isoform is respectively 69kDa, 56kDa and 31kDa. ATG16L2 may play a role in autophagy.

实验方案

Product Specific Protocols
WB protocol for ATG16L2 antibody 24322-1-APDownload protocol
IF protocol for ATG16L2 antibody 24322-1-APDownload protocol
IP protocol for ATG16L2 antibody 24322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Gene

Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.

Authors - Trieu-Duc Vu
View Article