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验证数据展示

  • IF Staining of MCF-7 using 24322-1-AP

    IF Staining of MCF-7 using 24322-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 24322-1-AP (ATG16L2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse spleen using 24322-1-AP

    WB analysis of mouse spleen using 24322-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 24322-1-AP (ATG16L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 24322-1-AP

    IP experiment of mouse testis using 24322-1-AP

    IP result of anti-ATG16L2 (IP:24322-1-AP, 3ug; Detection:24322-1-AP 1:700) with mouse testis tissue lysate 4800ug.

ATG16L2 Polyclonal antibody

ATG16L2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 24322-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

APG16 like 2, ATG16L2, Autophagy related protein 16 2, WDR80

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24322-1-AP targets ATG16L2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 ATG16L2 fusion protein Ag19399 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATG16 autophagy related 16-like 2 (S. cerevisiae)
别名 APG16 like 2, ATG16L2, Autophagy related protein 16 2, WDR80
计算分子量 619 aa, 69 kDa
观测分子量 70 kDa, 30 kDa
GenBank蛋白编号BC137490
基因名称 ATG16L2
Gene ID (NCBI) 89849
RRIDAB_2879493
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NAA4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG16L2 also named as Autophagy related protein 16-2 is a 619 amino-acid protein, Which belongs to the WD repeat ATG16 family. ATG16L2 contains 7 WD repeats and localizes in the cytoplasm. There are three isoforms of ATG16L2 and the molecular weight of three isoform is respectively 69kDa, 56kDa and 31kDa. ATG16L2 may play a role in autophagy.

实验方案

Product Specific Protocols
WB protocol for ATG16L2 antibody 24322-1-APDownload protocol
IF protocol for ATG16L2 antibody 24322-1-APDownload protocol
IP protocol for ATG16L2 antibody 24322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Gene

Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.

Authors - Trieu-Duc Vu
View Article