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ATG4A Polyclonal antibody

ATG4A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27467-1-AP

Print datasheet

Synonyms

APG4A, ATG4A, AUTL2, Autophagin 2, Cysteine protease ATG4A, hAPG4A

验证数据展示

  • WB analysis of L02 using 27467-1-AP

    WB analysis of L02 using 27467-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 27467-1-AP (ATG4A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 27467-1-AP

    IHC staining of human stomach cancer using 27467-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27467-1-AP (ATG4A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 27467-1-AP

    IHC staining of human stomach cancer using 27467-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27467-1-AP (ATG4A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inL02 cells, MKN-45 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27467-1-AP targets ATG4A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen ATG4A fusion protein Ag26497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG4 autophagy related 4 homolog A (S. cerevisiae)
Synonyms APG4A, ATG4A, AUTL2, Autophagin 2, Cysteine protease ATG4A, hAPG4A
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC061696
Gene Symbol ATG4A
Gene ID (NCBI) 115201
RRIDAB_2880879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYN0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG4, a member of the cysteine protease family, plays an important role in autophagy induction. ATG4 enzymes cleave the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine that is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes. ATG4A, which may be a target for cancer prevention and intervention, has been reported to be related to breast tumorigenesis, lung cancer, ovarian cancer, cervical cancer and gastric cancer. (PMID: 27276686, 29435029, 28636635)

实验方案

Product Specific Protocols
WB protocol for ATG4A antibody 27467-1-APDownload protocol
IHC protocol for ATG4A antibody 27467-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis.

Authors - Zhenhong Ni
View Article