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验证数据展示

  • WB analysis of L02 using 27467-1-AP

    WB analysis of L02 using 27467-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 27467-1-AP (ATG4A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 27467-1-AP

    IHC staining of human stomach cancer using 27467-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27467-1-AP (ATG4A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 27467-1-AP

    IHC staining of human stomach cancer using 27467-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27467-1-AP (ATG4A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATG4A Polyclonal antibody

ATG4A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27467-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

APG4A, ATG4A, AUTL2, Autophagin 2, Cysteine protease ATG4A, hAPG4A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, MKN-45 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1500
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27467-1-AP targets ATG4A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 ATG4A fusion protein Ag26497 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATG4 autophagy related 4 homolog A (S. cerevisiae)
别名 APG4A, ATG4A, AUTL2, Autophagin 2, Cysteine protease ATG4A, hAPG4A
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC061696
基因名称 ATG4A
Gene ID (NCBI) 115201
RRIDAB_2880879
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WYN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG4, a member of the cysteine protease family, plays an important role in autophagy induction. ATG4 enzymes cleave the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine that is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes. ATG4A, which may be a target for cancer prevention and intervention, has been reported to be related to breast tumorigenesis, lung cancer, ovarian cancer, cervical cancer and gastric cancer. (PMID: 27276686, 29435029, 28636635)

实验方案

Product Specific Protocols
WB protocol for ATG4A antibody 27467-1-APDownload protocol
IHC protocol for ATG4A antibody 27467-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis.

Authors - Zhenhong Ni
View Article