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验证数据展示

  • WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

    WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

    WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67727-1-Ig (same clone as 67727-1-PBS)

    IHC staining of human kidney using 67727-1-Ig (same clone as 67727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67727-1-Ig (same clone as 67727-1-PBS)

    IF Staining of HeLa using 67727-1-Ig (same clone as 67727-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATG4B antibody (67727-1-Ig, Clone: 1G5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • WB analysis of HuH-7 using 67727-1-Ig (same clone as 67727-1-PBS)

    WB analysis of HuH-7 using 67727-1-Ig (same clone as 67727-1-PBS)

    WB result of ATG4B antibody (67727-1-Ig; 1:800; room temperature for 1.5 hours) with wild-type and ATG4B knockout HuH-7 cells. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ATG4B Monoclonal antibody, PBS Only

ATG4B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67727-1-PBS

产品说明书

CloneNo. 1G5A7

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Autophagy-related cysteine endopeptidase 1, Autophagin-1, AUT-like 1 cysteine endopeptidase, AUTL1, APG4B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67727-1-PBS targets ATG4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATG4B fusion protein Ag30510 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ATG4 autophagy related 4 homolog B (S. cerevisiae)
别名 Autophagy-related cysteine endopeptidase 1, Autophagin-1, AUT-like 1 cysteine endopeptidase, AUTL1, APG4B
计算分子量 44 kDa
观测分子量44 kDa
GenBank蛋白编号BC000719
基因名称 ATG4B
Gene ID (NCBI) 23192
RRIDAB_2882913
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4P1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.