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  • Featured Product
  • KD/KO Validated

ATG4B Monoclonal antibody, PBS Only

ATG4B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5A7

Cat no : 67727-1-PBS

Print datasheet

Synonyms

Autophagy-related cysteine endopeptidase 1, Autophagin-1, AUT-like 1 cysteine endopeptidase, AUTL1, APG4B

验证数据展示

  • WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

    WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

    WB analysis using 67727-1-Ig (same clone as 67727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67727-1-Ig (ATG4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67727-1-Ig (same clone as 67727-1-PBS)

    IHC staining of human kidney using 67727-1-Ig (same clone as 67727-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67727-1-Ig (ATG4B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67727-1-Ig (same clone as 67727-1-PBS)

    IF Staining of HeLa using 67727-1-Ig (same clone as 67727-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATG4B antibody (67727-1-Ig, Clone: 1G5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67727-1-PBS in a different storage buffer formulation.

产品信息

67727-1-PBS targets ATG4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATG4B fusion protein Ag30510 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ATG4 autophagy related 4 homolog B (S. cerevisiae)
Synonyms Autophagy-related cysteine endopeptidase 1, Autophagin-1, AUT-like 1 cysteine endopeptidase, AUTL1, APG4B
Calculated Molecular Weight 44 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC000719
Gene Symbol ATG4B
Gene ID (NCBI) 23192
RRIDAB_2882913
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4P1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG4B(autophagy 4) is also named as APG4B, AUTL1, KIAA0943 and belongs to the peptidase C54 family. It is a homolog of yeast Apg4, a cysteine protease involved in autophagy and is a cytoplasmic enzyme. This protein is highly expressed in skeletal muscle, with lower expression in heart, liver, and pancreas and no expression is detected in fetal tissues by the northern blot. ATG4B is widely expressed in tumor cell lines (PMID:12446702). It has some isoforms produced by alternative splicing with molecular mass of 31-52 kDa.