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验证数据展示

  • WB analysis of Jurkat using 20382-1-AP

    WB analysis of Jurkat using 20382-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20382-1-AP (ATG4C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 20382-1-AP

    IHC staining of human heart using 20382-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 20382-1-AP

    IHC staining of human heart using 20382-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 20382-1-AP

    IF Staining of HeLa using 20382-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATG4C antibody (20382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ATG4C Polyclonal antibody

ATG4C Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 20382-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF, IHC, ELISA

别名

APG4 C, APG4C, ATG4C, AUTL1, AUTL3, Autophagin 3, Cysteine protease ATG4C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

20382-1-AP targets ATG4C in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 ATG4C fusion protein Ag14207 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATG4 autophagy related 4 homolog C (S. cerevisiae)
别名 APG4 C, APG4C, ATG4C, AUTL1, AUTL3, Autophagin 3, Cysteine protease ATG4C
计算分子量 458 aa, 52 kDa
观测分子量 52-55 kDa
GenBank蛋白编号BC033024
基因名称 ATG4C
Gene ID (NCBI) 84938
RRIDAB_10666852
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96DT6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATG4C antibody 20382-1-APDownload protocol
IHC protocol for ATG4C antibody 20382-1-APDownload protocol
IF protocol for ATG4C antibody 20382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Autophagy

Analysis of ATG4C function in vivo

Authors - Isaac Tamargo-Gómez
View Article
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun
View Article