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ATG4C Polyclonal antibody

ATG4C Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat No : 20382-1-AP

Print datasheet

Synonyms

APG4 C, APG4C, ATG4C, AUTL1, AUTL3, Autophagin 3, Cysteine protease ATG4C

验证数据展示

  • WB analysis of Jurkat using 20382-1-AP

    WB analysis of Jurkat using 20382-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20382-1-AP (ATG4C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 20382-1-AP

    IHC staining of human heart using 20382-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 20382-1-AP

    IHC staining of human heart using 20382-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 20382-1-AP

    IF Staining of HeLa using 20382-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATG4C antibody (20382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

20382-1-AP targets ATG4C in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen ATG4C fusion protein Ag14207 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG4 autophagy related 4 homolog C (S. cerevisiae)
Synonyms APG4 C, APG4C, ATG4C, AUTL1, AUTL3, Autophagin 3, Cysteine protease ATG4C
Calculated Molecular Weight 458 aa, 52 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC033024
Gene Symbol ATG4C
Gene ID (NCBI) 84938
RRIDAB_10666852
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATG4C antibody 20382-1-APDownload protocol
IHC protocol for ATG4C antibody 20382-1-APDownload protocol
IF protocol for ATG4C antibody 20382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Autophagy

Analysis of ATG4C function in vivo

Authors - Isaac Tamargo-Gómez
View Article
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun
View Article