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验证数据展示

  • WB analysis using 28413-1-AP

    WB analysis using 28413-1-AP

    HeLa cell lysates were subjected to SDS PAGE followed by western blot with 28413-1-AP (ATG7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28413-1-AP

    WB analysis of HeLa using 28413-1-AP

    WB result of ATG7 antibody (28413-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HeLa cells.

  • IHC staining of human cervical cancer using 28413-1-AP

    IHC staining of human cervical cancer using 28413-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 28413-1-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

ATG7 Polyclonal antibody

ATG7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28413-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

GSA7, Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28413-1-AP targets ATG7 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ATG7 fusion protein Ag29172 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATG7 autophagy related 7 homolog (S. cerevisiae)
别名 GSA7, Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L
计算分子量 78 kDa
观测分子量68-70 kDa
GenBank蛋白编号BC000091
基因名称 ATG7
Gene ID (NCBI) 10533
RRIDAB_2918161
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95352
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

实验方案

Product Specific Protocols
WB protocol for ATG7 antibody 28413-1-APDownload protocol
IHC protocol for ATG7 antibody 28413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols