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  • Featured Product
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ATG7 Polyclonal antibody

ATG7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28413-1-AP

Print datasheet

Synonyms

GSA7, Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L

验证数据展示

  • WB analysis using 28413-1-AP

    WB analysis using 28413-1-AP

    HeLa cell lysates were subjected to SDS PAGE followed by western blot with 28413-1-AP (ATG7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28413-1-AP

    WB analysis of HeLa using 28413-1-AP

    WB result of ATG7 antibody (28413-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HeLa cells.

  • IHC staining of human cervical cancer using 28413-1-AP

    IHC staining of human cervical cancer using 28413-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 28413-1-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28413-1-AP targets ATG7 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ATG7 fusion protein Ag29172 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Synonyms GSA7, Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L
Calculated Molecular Weight 78 kDa
Observed Molecular Weight68-70 kDa
GenBank Accession NumberBC000091
Gene Symbol ATG7
Gene ID (NCBI) 10533
RRIDAB_2918161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95352
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

实验方案

Product Specific Protocols
WB protocol for ATG7 antibody 28413-1-APDownload protocol
IHC protocol for ATG7 antibody 28413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols