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验证数据展示

  • WB analysis using 83817-1-RR

    WB analysis using 83817-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83817-1-RR (ATG8L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83817-1-RR

    IHC staining of mouse brain using 83817-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83817-1-RR (GABARAPL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 83817-1-RR

    IF Staining of HepG2 using 83817-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATG8L antibody (83817-1-RR, Clone: 240533F5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GABARAPL1 Recombinant antibody

GABARAPL1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83817-1-RR

产品说明书

CloneNo. 240533F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Early estrogen-regulated protein, ATG8L, ATG8, APG8L, APG8 LIKE

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse brain tissue, rat brain tissue, rat liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83817-1-RR targets GABARAPL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GABARAPL1 fusion protein Ag1473 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GABA(A) receptor-associated protein like 1
别名 Early estrogen-regulated protein, ATG8L, ATG8, APG8L, APG8 LIKE
计算分子量 14 kDa
观测分子量16 kDa
GenBank蛋白编号BC009309
基因名称 GABARAPL1
Gene ID (NCBI) 23710
RRIDAB_3671403
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H0R8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine.

实验方案

Product Specific Protocols
WB protocol for GABARAPL1 antibody 83817-1-RRDownload protocol
IHC protocol for GABARAPL1 antibody 83817-1-RRDownload protocol
IF protocol for GABARAPL1 antibody 83817-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols