验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, 3T3-L1 cells, NIH/3T3 cells, mouse heart tissue, mouse testis tissue, rat heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84736-1-RR targets ATGL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | patatin-like phospholipase domain containing 2 |
| 别名 | Adipose triglyceride lipase, PNPLA2, 241919C6, EC:3.1.1.4, EC:3.1.1.3 |
| 计算分子量 | 55 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | NM_020376 |
| 基因名称 | ATGL |
| Gene ID (NCBI) | 57104 |
| RRID | AB_3672120 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q96AD5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATGL antibody 84736-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |