验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, 3T3-L1 cells, NIH/3T3 cells, mouse heart tissue, mouse testis tissue, rat heart tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84736-1-RR targets ATGL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | patatin-like phospholipase domain containing 2 |
Synonyms | Adipose triglyceride lipase, PNPLA2, 241919C6, EC:3.1.1.4, EC:3.1.1.3 |
Calculated Molecular Weight | 55 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | NM_020376 |
Gene Symbol | ATGL |
Gene ID (NCBI) | 57104 |
RRID | AB_3672120 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q96AD5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.
实验方案
Product Specific Protocols | |
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WB protocol for ATGL antibody 84736-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |