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Species Reactivity
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验证数据展示

  • WB analysis using 84736-1-RR

    WB analysis using 84736-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84736-1-RR (ATGL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ATGL Recombinant antibody

ATGL Recombinant Antibody for WB, ELISA
Cat No. 84736-1-RR
CloneNo. 241919C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Adipose triglyceride lipase, PNPLA2, 241919C6, EC:3.1.1.4, EC:3.1.1.3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, 3T3-L1 cells, NIH/3T3 cells, mouse heart tissue, mouse testis tissue, rat heart tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84736-1-RR targets ATGL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name patatin-like phospholipase domain containing 2
Synonyms Adipose triglyceride lipase, PNPLA2, 241919C6, EC:3.1.1.4, EC:3.1.1.3
Calculated Molecular Weight 55 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberNM_020376
Gene Symbol ATGL
Gene ID (NCBI) 57104
RRIDAB_3672120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96AD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.

实验方案

Product Specific Protocols
WB protocol for ATGL antibody 84736-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols