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ATL3 Polyclonal antibody
ATL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse liver tissue, SMMC-7721 cells, Jurkat cells, NIH/3T3 cells, rat liver tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16921-1-AP targets ATL3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, monkey |
Immunogen | ATL3 fusion protein Ag10279 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | atlastin GTPase 3 |
Synonyms | EC:3.6.5.-, Atlastin-3, Atlastin 3 |
Calculated Molecular Weight | 541 aa, 61 kDa |
Observed Molecular Weight | 61 kDa |
GenBank Accession Number | BC077727 |
Gene Symbol | ATL3 |
Gene ID (NCBI) | 25923 |
RRID | AB_2290228 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q6DD88 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ATL3 antibody 16921-1-AP | Download protocol |
IHC protocol for ATL3 antibody 16921-1-AP | Download protocol |
IF protocol for ATL3 antibody 16921-1-AP | Download protocol |
IP protocol for ATL3 antibody 16921-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Genes Dev Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein | ||
Elife Efficient protein targeting to the inner nuclear membrane requires Atlastin-dependent maintenance of ER topology.
| ||
Curr Biol ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.
| ||
Nat Commun Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites. |