验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ATM Ready-To-Use IHC Kit. ATM primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
67586-1-Ig targets ATM in WB, IF, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, rat, pig |
| Immunogen | ATM fusion protein Ag30001 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ataxia telangiectasia mutated |
| Synonyms | Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated, 3E6F4 |
| Observed Molecular Weight | 310-350 kDa |
| GenBank Accession Number | BC137169 |
| Gene Symbol | ATM |
| Gene ID (NCBI) | 472 |
| RRID | AB_2882795 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13315 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATM antibody 67586-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Food Chem Toxicol DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells | ||
CNS Neurosci Ther Multiple sevoflurane exposures during mid-trimester induce neurotoxicity in the developing brain initiated by 15LO2-Mediated ferroptosis | ||
J Mol Med (Berl) High expression of BCAT1 sensitizes AML cells to PARP inhibitor by suppressing DNA damage response | ||
Signal Transduct Target Ther In vivo evaluation of guide-free Cas9-induced safety risks in a pig model |
