验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:5000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IF | See 1 publications below |
产品信息
67586-1-Ig targets ATM in WB, IF, ELISA applications and shows reactivity with human, rat samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, rat |
文献引用反应性 | human, rat, pig |
免疫原 | ATM fusion protein Ag30001 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | ataxia telangiectasia mutated |
别名 | Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated, 3E6F4 |
观测分子量 | 310-350 kDa |
GenBank蛋白编号 | BC137169 |
基因名称 | ATM |
Gene ID (NCBI) | 472 |
RRID | AB_2882795 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q13315 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.
实验方案
Product Specific Protocols | |
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WB protocol for ATM antibody 67586-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Food Chem Toxicol DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells | ||
CNS Neurosci Ther Multiple sevoflurane exposures during mid-trimester induce neurotoxicity in the developing brain initiated by 15LO2-Mediated ferroptosis | ||
J Mol Med (Berl) High expression of BCAT1 sensitizes AML cells to PARP inhibitor by suppressing DNA damage response | ||
Signal Transduct Target Ther In vivo evaluation of guide-free Cas9-induced safety risks in a pig model |