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ATM Recombinant antibody, PBS Only (Detector)

ATM Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240560H8

Cat no : 83608-4-PBS

Print datasheet

Synonyms

Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated, 240560H8

验证数据展示

  • IF Staining of HeLa using 83608-4-RR (same clone as 83608-4-PBS)

    IF Staining of HeLa using 83608-4-RR (same clone as 83608-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATM antibody (83608-4-RR, Clone: 240560H8 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83608-4-RR (same clone as 83608-4-PBS)

    FC experiment of HeLa using 83608-4-RR (same clone as 83608-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATM Recombinant Antibody (83608-4-RR, Clone:240560H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00592-4

    Sandwich ELISA standard curve of MP00592-4

    Sandwich ELISA standard curve of MP00592-4, Human ATM Recombinant Matched Antibody Pair - PBS only. 83608-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25920. 83608-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP00592-4

    Sample test of MP00592-4

    The mean ATM concentration was determined to be 3,383.80 pg/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load and 3,536.69 pg/mL in MDA-MB-468 cell extract based on a 3.20 mg/mL extract load.

  • Cytometric bead array standard curve of MP00592-1

    Cytometric bead array standard curve of MP00592-1

    Cytometric bead array standard curve of MP00592-1, ATM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83608-1-PBS. Detection antibody: 83608-4-PBS. Standard: Ag25920. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00592-3

    Cytometric bead array standard curve of MP00592-3

    Cytometric bead array standard curve of MP00592-3, ATM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83608-2-PBS. Detection antibody: 83608-4-PBS. Standard: Ag25920. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83608-4-RR

    Affinity and Kinetic Characterization of 83608-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM.

Planning an IHC experiment? We recommend our IHCeasy ATM Ready-To-Use IHC Kit. ATM primary antibody included.

产品信息

83608-4-PBS targets ATM as part of a matched antibody pair:

MP00592-1: 83608-1-PBS capture and 83608-4-PBS detection (validated in Cytometric bead array)

MP00592-3: 83608-2-PBS capture and 83608-4-PBS detection (validated in Cytometric bead array)

MP00592-4: 83608-6-PBS capture and 83608-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen ATM fusion protein Ag25920 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ataxia telangiectasia mutated
Synonyms Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated, 240560H8
GenBank Accession NumberBC137169
Gene Symbol ATM
Gene ID (NCBI) 472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13315
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C