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ATM Recombinant antibody, PBS Only

ATM Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240560F5

Cat no : 83608-5-PBS

Print datasheet

Synonyms

Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated, 240560F5

验证数据展示

  • WB analysis using 83608-5-RR (same clone as 83608-5-PBS)

    WB analysis using 83608-5-RR (same clone as 83608-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83608-5-RR (ATM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

    IF Staining of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83608-5-RR (same clone as 83608-5-PBS)

    IF Staining of HepG2 using 83608-5-RR (same clone as 83608-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83608-5-RR (same clone as 83608-5-PBS)

    IF Staining of HeLa using 83608-5-RR (same clone as 83608-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

    FC experiment of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ATM Recombinant antibody (83608-5-RR, Clone:240560F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83608-5-RR

    Affinity and Kinetic Characterization of 83608-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-5-RR against Human ATM were performed. The affinity constant is 0.318 nM.

Planning an IHC experiment? We recommend our IHCeasy ATM Ready-To-Use IHC Kit. ATM primary antibody included.

产品信息

83608-5-PBS targets ATM in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ATM fusion protein Ag25920 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ataxia telangiectasia mutated
Synonyms Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated, 240560F5
Observed Molecular Weight 310-350 kDa
GenBank Accession NumberBC137169
Gene Symbol ATM
Gene ID (NCBI) 472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13315
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.