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ATN1 Polyclonal antibody

ATN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19243-1-AP

Print datasheet

Synonyms

ATN1, atrophin 1, B37, D12S755E, DRPLA, HRS, NOD

验证数据展示

  • WB analysis of mouse testis using 19243-1-AP

    WB analysis of mouse testis using 19243-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19243-1-AP (ATN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 19243-1-AP

    IHC staining of human ovary using 19243-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19243-1-AP (ATN1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 19243-1-AP

    IHC staining of human ovary using 19243-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19243-1-AP (ATN1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman ovary tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19243-1-AP targets ATN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATN1 fusion protein Ag5835 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name atrophin 1
Synonyms ATN1, atrophin 1, B37, D12S755E, DRPLA, HRS, NOD
Calculated Molecular Weight 125 kDa
Observed Molecular Weight 105-110 kDa
GenBank Accession NumberBC051795
Gene Symbol ATN1
Gene ID (NCBI) 1822
RRIDAB_2878575
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATN1 also known as Atrophin-1 is a protein that in humans is encoded by the ATN1 gene. Dentatorubral pallidoluysian atrophy is a rare neurodegenerative disorder characterized by cerebellar ataxia, myoclonic epilepsy, choreoathetosis, and dementia. The disorder is related to the expansion of a trinucleotide repeat within this gene. The encoded protein includes a serine repeat and a region of alternating acidic and basic amino acids, as well as the variable glutamine repeat.

实验方案

Product Specific Protocols
WB protocol for ATN1 antibody 19243-1-APDownload protocol
IHC protocol for ATN1 antibody 19243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols