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  • Featured Product
  • KD/KO Validated

ATP11B Polyclonal antibody

ATP11B Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 13672-1-AP

Print datasheet

Synonyms

Phospholipid-transporting ATPase IF, P4-ATPase flippase complex alpha subunit ATP11B, EC:7.6.2.1, ATPIR, ATPIF

验证数据展示

  • IHC staining of human ovary tumor using 13672-1-AP

    IHC staining of human ovary tumor using 13672-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13672-1-AP (ATP11B Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human ovary tumor using 13672-1-AP

    IHC staining of human ovary tumor using 13672-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13672-1-AP (ATP11B Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HEK-293 using 13672-1-AP

    IF Staining of HEK-293 using 13672-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP11B antibody (13672-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13672-1-AP targets ATP11B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ATP11B fusion protein Ag4654 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, class VI, type 11B
Synonyms Phospholipid-transporting ATPase IF, P4-ATPase flippase complex alpha subunit ATP11B, EC:7.6.2.1, ATPIR, ATPIF
Calculated Molecular Weight1177aa, 134 kDa
GenBank Accession NumberBC033880
Gene Symbol ATP11B
Gene ID (NCBI) 23200
RRIDAB_2063150
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2G3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ATP11B antibody 13672-1-APDownload protocol
IF protocol for ATP11B antibody 13672-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Acta Neuropathol

Loss of the heterogeneous expression of flippase ATP11B leads to cerebral small vessel disease in a normotensive rat model.

Authors - Sophie Quick
View Article
human,mouseWB,IHC

J Immunother Cancer

Oncolytic peptide LTX-315 induces anti-pancreatic cancer immunity by targeting the ATP11B-PD-L1 axis.

Authors - Tianyu Tang
  • KO Validated
View Article
ratWB

Life Sci

miRNA-150-5p associate with antihypertensive effect of epigallocatechin-3-gallate revealed by aorta miRNome analysis of spontaneously hypertensive rat.

Authors - Bing-Jun Qian
View Article