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验证数据展示

  • WB analysis using 68505-1-Ig

    WB analysis using 68505-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of SK-BR-3 using 68505-1-Ig

    WB analysis of SK-BR-3 using 68505-1-Ig

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat kidney using 68505-1-Ig

    WB analysis of rat kidney using 68505-1-Ig

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig brain using 68505-1-Ig

    WB analysis of pig brain using 68505-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of C2C12 using 68505-1-Ig

    IF Staining of C2C12 using 68505-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ATP1A1/2 antibody (68505-1-Ig, Clone: 3D8G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

ATP1A1/2 Monoclonal antibody

ATP1A1/2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68505-1-Ig

产品说明书

CloneNo. 3D8G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

ATP1A2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2, alpha 2 ATPase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, SK-BR-3 cells, rat kidney tissue, pig brain tissue, mouse brain tissue
Positive IF/ICC detected inC2C12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68505-1-Ig targets ATP1A1/2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ATP1A1/2 fusion protein Ag10489 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
别名 ATP1A2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2, alpha 2 ATPase
计算分子量 1020 aa, 112 kDa
观测分子量 97-100 kDa
GenBank蛋白编号BC052271
基因名称 ATP1A2
Gene ID (NCBI) 477
RRIDAB_3085215
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50993
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2.

实验方案

Product Specific Protocols
WB protocol for ATP1A1/2 antibody 68505-1-IgDownload protocol
IF protocol for ATP1A1/2 antibody 68505-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols