ATP1A3 Polyclonal antibody
ATP1A3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue |
Positive IP detected in | rat brain tissue |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 1 publications below |
产品信息
25727-1-AP targets ATP1A3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Immunogen | ATP1A3 fusion protein Ag22842 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ATPase, Na+/K+ transporting, alpha 3 polypeptide |
Synonyms | ATP1A3, DYT12, RDP, Sodium pump subunit alpha 3 |
Calculated Molecular Weight | 1013 aa, 112 kDa |
Observed Molecular Weight | 110-113 kDa |
GenBank Accession Number | BC015566 |
Gene Symbol | ATP1A3 |
Gene ID (NCBI) | 478 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P13637 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ATP1A3 antibody 25727-1-AP | Download protocol |
IHC protocol for ATP1A3 antibody 25727-1-AP | Download protocol |
IF protocol for ATP1A3 antibody 25727-1-AP | Download protocol |
IP protocol for ATP1A3 antibody 25727-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Commun Biol PEX3 promotes regenerative repair after myocardial injury in mice through facilitating plasma membrane localization of ITGB3 | ||
Dis Model Mech HBS1L deficiency causes retinal dystrophy in a child and a mouse model associated with defective development of photoreceptor cells |