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Species Reactivity
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验证数据展示

  • WB analysis of C2C12 using 10868-1-AP (same clone as 10868-1-PBS)

    WB analysis of C2C12 using 10868-1-AP (same clone as 10868-1-PBS)

    C2C12 cell were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • WB analysis using 10868-1-AP (same clone as 10868-1-PBS)

    WB analysis using 10868-1-AP (same clone as 10868-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • WB analysis using 10868-1-AP (same clone as 10868-1-PBS)

    WB analysis using 10868-1-AP (same clone as 10868-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10868-1-AP (same clone as 10868-1-PBS)

    IHC staining of human prostate cancer using 10868-1-AP (same clone as 10868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP1A3 antibody included
  • IHC staining of human prostate cancer using 10868-1-AP (same clone as 10868-1-PBS)

    IHC staining of human prostate cancer using 10868-1-AP (same clone as 10868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP1A3 antibody included
  • IF Staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

    IF Staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

    IF Staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

ATP1A3 (C-terminal) Polyclonal antibody, PBS Only

ATP1A3 (C-terminal) Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 10868-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ATP1A3 Ready-To-Use IHC Kit. ATP1A3 primary antibody included.

产品信息

10868-1-PBS targets ATP1A3 (C-terminal) in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATP1A3 (C-terminal) fusion protein Ag1313 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, alpha 3 polypeptide
别名
计算分子量 113 kDa
观测分子量 100-113 kDa
GenBank蛋白编号BC015566
基因名称 ATP1A3
Gene ID (NCBI) 478
RRIDAB_2060962
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13637
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na+/K+ homeostasis. This antibody is generated against the C-terminal region (665-1013aa) of ATP1A3 and detects the band around 100-113 kDa in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)