ATP1B2 Polyclonal antibody

ATP1B2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 22338-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA and More (1)

Adhesion molecule in glia, ATPase Na+/K+ beta 2, Sodium/potassium-dependent ATPase subunit beta-2, Sodium/potassium-transporting ATPase subunit beta-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, C6 cells, C2C12 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22338-1-AP targets ATP1B2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 ATP1B2 fusion protein Ag17818 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, beta 2 polypeptide
别名 Adhesion molecule in glia, ATPase Na+/K+ beta 2, Sodium/potassium-dependent ATPase subunit beta-2, Sodium/potassium-transporting ATPase subunit beta-2
计算分子量 290 aa, 33 kDa
观测分子量 45-65 kDa
GenBank蛋白编号BC126175
基因名称 ATP1B2
Gene ID (NCBI) 482
RRIDAB_2879077
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14415
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1B2 is the β2 subunit of Na+/K+-ATPase which is an essential membrane-bound enzyme responsible for the transport of Na+ and K+ in most eukaryotic cells. ATP1B2 is also called the adhesion molecule on glia (AMOG) and it is highly expressed in normal glia. It is a heavily glycosylated protein that plays a role in cellular adhesion in the CNS. Recently differential expression of ATP1B2 has been found in some glioneuronal tumors (PMID: 23887941, 19371356). This antibody recognizes the endogenous ATP1B2 protein in human brain. The bands between 45 kDa and 65 kDa represent the glycosylated forms of ATP1B2 in different levels (PMID: 8918259).

实验方案

Product Specific Protocols
WB protocol for ATP1B2 antibody 22338-1-APDownload protocol
IHC protocol for ATP1B2 antibody 22338-1-APDownload protocol
IF protocol for ATP1B2 antibody 22338-1-APDownload protocol
IP protocol for ATP1B2 antibody 22338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Mol Gastroenterol Hepatol

Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells.

Authors - Yuli Wang
ratWB

Redox Biol

Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration.

Authors - Xiaoying Luo
humanWB

Cancer Cell Int

Spider venom components decrease glioblastoma cell migration and invasion through RhoA-ROCK and Na+/K+-ATPase β2: potential molecular entities to treat invasive brain cancer.

Authors - Natália Barreto
human,ratWB

World J Biol Psychiatry

Altered expression levels of miR-144-3p and ATP1B2 are associated with schizophrenia.

Authors - Bo Pan
humanWB

Eur J Appl Physiol

Resistance training upregulates skeletal muscle Na+, K+-ATPase content, with elevations in both α1 and α2, but not β isoforms.

Authors - Muath M Altarawneh
ratWB

Neurosci Lett

Effects of icariin on alleviating schizophrenia-like symptoms by regulating the miR-144-3p/ATP1B2/mTOR signalling pathway

Authors - Bo Pan
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