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ATP1B3 Polyclonal antibody

ATP1B3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11142-1-AP

Print datasheet

Synonyms

ATP1B3, ATPB 3, CD298

验证数据展示

  • WB analysis using 11142-1-AP

    WB analysis using 11142-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11142-1-AP (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11142-1-AP

    IHC staining of human prostate cancer using 11142-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11142-1-AP (ATP1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1B3 antibody included
  • IHC staining of human prostate cancer using 11142-1-AP

    IHC staining of human prostate cancer using 11142-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11142-1-AP (ATP1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1B3 antibody included

经过测试的应用

Positive WB detected inJurkat cells, THP-1 cells
Positive IHC detected inhuman prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ATP1B3 Ready-To-Use IHC Kit. ATP1B3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11142-1-AP targets ATP1B3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen ATP1B3 fusion protein Ag1589 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Na+/K+ transporting, beta 3 polypeptide
Synonyms ATP1B3, ATPB 3, CD298
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 38-43 kDa
GenBank Accession NumberBC011835
Gene Symbol ATP1B3
Gene ID (NCBI) 483
RRIDAB_2274459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54709
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

实验方案

Product Specific Protocols
WB protocol for ATP1B3 antibody 11142-1-APDownload protocol
IHC protocol for ATP1B3 antibody 11142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis

Authors - Fangquan Chen
View Article