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验证数据展示

  • WB analysis using 30035-1-AP

    WB analysis using 30035-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30035-1-AP (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 30035-1-AP

    IHC staining of human colon using 30035-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30035-1-AP (ATP2B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP2B1 antibody included
  • IHC staining of human pancreas cancer using 30035-1-AP

    IHC staining of human pancreas cancer using 30035-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30035-1-AP (ATP2B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP2B1 antibody included

ATP2B1 Polyclonal antibody

ATP2B1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30035-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

ATP2B1, PMCA1, PMCA1kb

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in37°C incubated mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ATP2B1 Ready-To-Use IHC Kit. ATP2B1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30035-1-AP targets ATP2B1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 ATP2B1 fusion protein Ag32423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, Ca++ transporting, plasma membrane 1
别名 ATP2B1, PMCA1, PMCA1kb
计算分子量135 kDa
观测分子量130-180 kDa
GenBank蛋白编号NM_001001323.1
基因名称 ATP2B1
Gene ID (NCBI) 490
RRIDAB_3086214
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20020
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).

实验方案

Product Specific Protocols
WB protocol for ATP2B1 antibody 30035-1-APDownload protocol
IHC protocol for ATP2B1 antibody 30035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols