验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, HEK-293 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
19678-1-AP targets PMCA2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, chicken |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, Ca++ transporting, plasma membrane 2 |
| 别名 | ATP2B2, PMCA2, PMCA2a, PMCA2i |
| 计算分子量 | 139 kDa |
| 观测分子量 | 137 kDa |
| GenBank蛋白编号 | NM_001001331 |
| 基因名称 | PMCA2 |
| Gene ID (NCBI) | 491 |
| RRID | AB_10644327 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01814 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP2B2, also named as PMCA2, belongs to the cation transport ATPase (P-type) family and type IIB subfamily. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. ATG2B2 catalyze the reaction:ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PMCA2 antibody 19678-1-AP | Download protocol |
| IHC protocol for PMCA2 antibody 19678-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Sci Rapid affinity purification of intracellular organelles using a twin strep tag. | ||
Metallomics Thioredoxin silencing-induced cardiac supercontraction occurs through endoplasmic reticulum stress and calcium overload in chicken. | ||
Reprod Domest Anim Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation. | ||
Mol Ther Nucleic Acids lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway. |
