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PMCA2 Polyclonal antibody

PMCA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19678-1-AP

Print datasheet

Synonyms

ATP2B2, PMCA2, PMCA2a, PMCA2i

验证数据展示

  • WB analysis of human brain using 19678-1-AP

    WB analysis of human brain using 19678-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19678-1-AP (PMCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19678-1-AP

    IHC staining of human brain using 19678-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19678-1-AP (PMCA2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 19678-1-AP

    IHC staining of human brain using 19678-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19678-1-AP (PMCA2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

19678-1-AP targets PMCA2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Ca++ transporting, plasma membrane 2
Synonyms ATP2B2, PMCA2, PMCA2a, PMCA2i
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 137 kDa
GenBank Accession NumberNM_001001331
Gene Symbol PMCA2
Gene ID (NCBI) 491
RRIDAB_10644327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01814
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP2B2, also named as PMCA2, belongs to the cation transport ATPase (P-type) family and type IIB subfamily. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. ATG2B2 catalyze the reaction:ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).

实验方案

Product Specific Protocols
WB protocol for PMCA2 antibody 19678-1-APDownload protocol
IHC protocol for PMCA2 antibody 19678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Sci

Rapid affinity purification of intracellular organelles using a twin strep tag.

Authors - Jian Xiong
View Article
chickenWB

Metallomics

Thioredoxin silencing-induced cardiac supercontraction occurs through endoplasmic reticulum stress and calcium overload in chicken.

Authors - Jie Yang
View Article
WB

Reprod Domest Anim

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.

Authors - M D Xi
View Article
WB

Mol Ther Nucleic Acids

lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway.

Authors - Jie Yang
View Article