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验证数据展示

  • WB analysis using 18692-1-AP

    WB analysis using 18692-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 18692-1-AP

    IHC staining of human stomach cancer using 18692-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 18692-1-AP (ATP4B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse stomach using 18692-1-AP

    IF Staining of mouse stomach using 18692-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using ATP4B antibody (18692-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP4B Polyclonal antibody

ATP4B Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 18692-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA and More (1)

别名

Potassium-transporting ATPase subunit beta, H+/K+ ATPase beta, ATP4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse stomach tissue, mouse colon tissue, rat kidney tissue, rat stomach
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse stomach tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

18692-1-AP targets ATP4B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 ATP4B fusion protein Ag6935 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, H+/K+ exchanging, beta polypeptide
别名 Potassium-transporting ATPase subunit beta, H+/K+ ATPase beta, ATP4
计算分子量 33 kDa
观测分子量 60-80 kDa
GenBank蛋白编号BC029059
基因名称 ATP4B
Gene ID (NCBI) 496
RRIDAB_10597241
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51164
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATP4B antibody 18692-1-APDownload protocol
IHC protocol for ATP4B antibody 18692-1-APDownload protocol
IF protocol for ATP4B antibody 18692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

FASEB J

Palmatine ameliorates N-methyl-N'-nitrosoguanidine-induced chronic atrophic gastritis through the STAT1/CXCL10 axis

Authors - Yuan Zhou
View Article