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ATP4B Polyclonal antibody

ATP4B Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18692-1-AP

Print datasheet

Synonyms

Potassium-transporting ATPase subunit beta, H+/K+ ATPase beta, ATP4

验证数据展示

  • WB analysis using 18692-1-AP

    WB analysis using 18692-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 18692-1-AP

    IHC staining of human stomach cancer using 18692-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 18692-1-AP (ATP4B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse stomach using 18692-1-AP

    IF Staining of mouse stomach using 18692-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using ATP4B antibody (18692-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse stomach tissue, mouse colon tissue, rat kidney tissue, rat stomach
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse stomach tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

18692-1-AP targets ATP4B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen ATP4B fusion protein Ag6935 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, H+/K+ exchanging, beta polypeptide
Synonyms Potassium-transporting ATPase subunit beta, H+/K+ ATPase beta, ATP4
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 60-80 kDa
GenBank Accession NumberBC029059
Gene Symbol ATP4B
Gene ID (NCBI) 496
RRIDAB_10597241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51164
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATP4B antibody 18692-1-APDownload protocol
IHC protocol for ATP4B antibody 18692-1-APDownload protocol
IF protocol for ATP4B antibody 18692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

FASEB J

Palmatine ameliorates N-methyl-N'-nitrosoguanidine-induced chronic atrophic gastritis through the STAT1/CXCL10 axis

Authors - Yuan Zhou
View Article