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验证数据展示

  • WB analysis using 15408-1-AP

    WB analysis using 15408-1-AP

    Boiled and 37℃ incubated mouse heart tissue were subjected to SDS PAGE followed by western blot with 15408-1-AP (ATP5E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15408-1-AP

    IF Staining of HeLa using 15408-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 15408-1-AP

    IF Staining of HeLa using 15408-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ATP5E Polyclonal antibody

ATP5E Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15408-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

ATPE, ATP5F1E, ATP synthase F1 subunit epsilon

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, 37℃ incubated mouse heart tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15408-1-AP targets ATP5E in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATP5E fusion protein Ag7672 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
Synonyms ATPE, ATP5F1E, ATP synthase F1 subunit epsilon
Calculated Molecular Weight 6 kDa
Observed Molecular Weight6 kDa
GenBank Accession NumberBC001690
Gene Symbol ATP5E
Gene ID (NCBI) 514
RRIDAB_3085461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56381
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP synthase epsilon subunit (ATP5E) is an important subunit of ATP synthase, which is located in the stalk region of the F1 sector. F1-ATPase is the catalytic portion of mitochondrial ATP synthase, which produces ATP from ADP and inorganic phosphate (Pi). ATP5E is widely expressed in tissues. The calculated molecular weight of ATP5E is 6 kDa (PMID: 36625260).

实验方案

Product Specific Protocols
WB protocol for ATP5E antibody 15408-1-APDownload protocol
IF protocol for ATP5E antibody 15408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols