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验证数据展示

  • WB analysis using 15408-1-AP

    WB analysis using 15408-1-AP

    Boiled and 37℃ incubated mouse heart tissue were subjected to SDS PAGE followed by western blot with 15408-1-AP (ATP5E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15408-1-AP

    IF Staining of HeLa using 15408-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 15408-1-AP

    IF Staining of HeLa using 15408-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5E antibody (15408-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ATP5E Polyclonal antibody

ATP5E Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15408-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

ATPE, ATP5F1E, ATP synthase F1 subunit epsilon

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, 37℃ incubated mouse heart tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15408-1-AP targets ATP5E in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ATP5E fusion protein Ag7672 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
别名 ATPE, ATP5F1E, ATP synthase F1 subunit epsilon
计算分子量 6 kDa
观测分子量6 kDa
GenBank蛋白编号BC001690
基因名称 ATP5E
Gene ID (NCBI) 514
RRIDAB_3085461
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56381
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP synthase epsilon subunit (ATP5E) is an important subunit of ATP synthase, which is located in the stalk region of the F1 sector. F1-ATPase is the catalytic portion of mitochondrial ATP synthase, which produces ATP from ADP and inorganic phosphate (Pi). ATP5E is widely expressed in tissues. The calculated molecular weight of ATP5E is 6 kDa (PMID: 36625260).

实验方案

Product Specific Protocols
WB protocol for ATP5E antibody 15408-1-APDownload protocol
IF protocol for ATP5E antibody 15408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols