ATP5H Polyclonal antibody

ATP5H Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17589-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

My032, ATPQ, ATP5PD, ATP5JD, ATP5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, mouse liver tissue, rat brain tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17589-1-AP targets ATP5H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 ATP5H fusion protein Ag11429 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
别名 My032, ATPQ, ATP5PD, ATP5JD, ATP5
计算分子量 137 aa, 16 kDa
观测分子量 19-22 kDa
GenBank蛋白编号BC032245
基因名称 ATP5H
Gene ID (NCBI) 10476
RRIDAB_2062046
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75947
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.

实验方案

Product Specific Protocols
WB protocol for ATP5H antibody 17589-1-APDownload protocol
IHC protocol for ATP5H antibody 17589-1-APDownload protocol
IF protocol for ATP5H antibody 17589-1-APDownload protocol
IP protocol for ATP5H antibody 17589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Brain

Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy.

Authors - Xia Feng
mouseWB

Theranostics

AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages.

Authors - Liangjun Xia
humanIHC

Brain Pathol

Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus.

Authors - Margalida A Frau-Méndez
humanWB

Oncol Lett

Dihydroartemisinin inhibits liver cancer cell migration and invasion by reducing ATP synthase production through CaMKK2/NCLX

Authors - Jiang Chang
mouseWB

J Nanobiotechnology

Modulation of alveolar macrophage and mitochondrial fitness by medicinal plant-derived nanovesicles to mitigate acute lung injury and viral pneumonia

Authors - Lusha Ye
human,mouseWB

Science

Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists

Authors - Gregory A Wyant
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