验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15842-1-AP targets ATP5J2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ATP5J2 fusion protein Ag8598 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2 |
| 别名 | ATP5MF, ATP5JL, ATP synthase membrane subunit f |
| 计算分子量 | 5-11 kDa |
| 观测分子量 | 11kDa |
| GenBank蛋白编号 | BC003678 |
| 基因名称 | ATP5J2 |
| Gene ID (NCBI) | 9551 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56134 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP5J2 (also known as ATP synthase-coupling factor 6, or CF6) is a nuclear-encoded subunit of mitochondrial ATP synthase (Complex V), the enzyme responsible for synthesizing ATP from ADP and inorganic phosphate (Pi) during oxidative phosphorylation (OXPHOS).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP5J2 antibody 15842-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |