ATP6 Monoclonal antibody
ATP6 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1H4G5
验证数据展示
经过测试的应用
| Positive WB detected in | rat cerebellum tissue, Rat heart tissue, Rat liver tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68442-1-Ig targets ATP6 in WB, ELISA applications and shows reactivity with Human, Rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, Rat |
| Cited Reactivity | rat |
| Immunogen | ATP6 fusion protein Ag31940 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ATP synthase 6; ATPase subunit 6 |
| Synonyms | ATP synthase subunit a, ATP6, ATPASE6, F ATPase protein 6, MT ATP6, MTATP6 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | YP_003024031 |
| Gene Symbol | ATP6 |
| Gene ID (NCBI) | 4508 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P00846 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP synthase, also known as FoF1 complex, is a critical mitochondrial OXPHOS enzyme involved in the regulation of mitochondrial ATP production and in the maintenance of the mitochondrial membrane potential. It is composed of three components (F1, Fo and the peripheral stalk).
F1, the soluble catalytic core, is above the membrane, inside the matrix of the mitochondria; consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon); Fo, comprising the proton channel, is within the membrane; Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP6 is the a subunit of Fo region.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6 antibody 68442-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hazard Mater ZLN005 alleviates PBDE-47 induced impairment of mitochondrial translation and neurotoxicity through PGC-1α/ERRα axis |