ATP6V0A1 Polyclonal antibody
ATP6V0A1 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13828-1-AP targets ATP6V0A1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | ATP6V0A1 fusion protein Ag4191 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ATPase, H+ transporting, lysosomal V0 subunit a1 |
| Synonyms | V-ATPase 116 kDa subunit a 1, Stv1, Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, ATP6N1A, ATP6N1 |
| Calculated Molecular Weight | 837 aa, 96 kDa |
| Observed Molecular Weight | 116 kDa |
| GenBank Accession Number | BC032398 |
| Gene Symbol | ATP6V0A1 |
| Gene ID (NCBI) | 535 |
| RRID | AB_2877979 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q93050 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation(PMID: 7774924 34904569).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6V0A1 antibody 13828-1-AP | Download protocol |
| IHC protocol for ATP6V0A1 antibody 13828-1-AP | Download protocol |
| IP protocol for ATP6V0A1 antibody 13828-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. | ||
Int J Biol Sci Toosendanin, a novel potent vacuolar-type H+-translocating ATPase inhibitor, sensitizes cancer cells to chemotherapy by blocking protective autophagy. | ||
J Cell Physiol A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells. | ||
Mol Immunol Systematic deletion of adenosine receptors reveals novel roles in inflammation and pyroptosis in THP-1 macrophages. | ||
Cell Death Dis PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming. |
