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  • Featured Product
  • KD/KO Validated

ATP6V1A Monoclonal antibody, PBS Only

ATP6V1A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C5G2

Cat no : 68440-1-PBS

Print datasheet

Synonyms

V-ATPase 69 kDa subunit, VA68, EC:7.1.2.2, ATP6V1A1, ATP6A1

验证数据展示

  • WB analysis using 68440-1-Ig (same clone as 68440-1-PBS)

    WB analysis using 68440-1-Ig (same clone as 68440-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68440-1-Ig (ATP6V1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68440-1-Ig (same clone as 68440-1-PBS)

    WB analysis of HeLa using 68440-1-Ig (same clone as 68440-1-PBS)

    WB result of ATP6V1A antibody (68440-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1A transfected HeLa cells. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

    IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

    IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

产品信息

68440-1-PBS targets ATP6V1A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen ATP6V1A fusion protein Ag10820 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Synonyms V-ATPase 69 kDa subunit, VA68, EC:7.1.2.2, ATP6V1A1, ATP6A1
Calculated Molecular Weight 617 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC013138
Gene Symbol ATP6V1A
Gene ID (NCBI) 523
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP38606
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.