验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, human brain tissue, human testis tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IHC | See 1 publications below |
产品信息
16054-1-AP targets ATP6V1C1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ATP6V1C1 fusion protein Ag9020 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1 |
| 别名 | ATP6C, ATP6D, ATP6V1C1, V ATPase subunit C 1, VATC, Vma5 |
| 计算分子量 | 382 aa, 44 kDa |
| 观测分子量 | 42-44 kDa |
| GenBank蛋白编号 | BC010960 |
| 基因名称 | ATP6V1C1 |
| Gene ID (NCBI) | 528 |
| RRID | AB_2062501 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21283 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6V1C1 antibody 16054-1-AP | Download protocol |
| IHC protocol for ATP6V1C1 antibody 16054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem A brain-enriched Drp1 isoform associates with lysosomes, late endosomes and the plasma membrane. | ||
Commun Biol A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets. | ||
Biochem Biophys Res Commun Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells. | ||
Bioengineered A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma. | ||
Autophagy Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate |
