ATP6V1C1 Polyclonal antibody

ATP6V1C1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16054-1-AP

Print datasheet

Synonyms

ATP6C, ATP6D, ATP6V1C1, V ATPase subunit C 1, VATC, Vma5



经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, human brain tissue, human testis tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16054-1-AP targets ATP6V1C1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ATP6V1C1 fusion protein Ag9020 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
Synonyms ATP6C, ATP6D, ATP6V1C1, V ATPase subunit C 1, VATC, Vma5
Calculated Molecular Weight 382 aa, 44 kDa
Observed Molecular Weight 42-44 kDa
GenBank Accession NumberBC010960
Gene Symbol ATP6V1C1
Gene ID (NCBI) 528
RRIDAB_2062501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21283
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATP6V1C1 antibody 16054-1-APDownload protocol
IHC protocol for ATP6V1C1 antibody 16054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Ther

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Authors - Benfu Zhong
mouseWB

J Biol Chem

A brain-enriched Drp1 isoform associates with lysosomes, late endosomes and the plasma membrane.

Authors - Kie Itoh
humanWB

Commun Biol

A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets.

Authors - Masashi Watanabe
humanWB

Biochem Biophys Res Commun

Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.

Authors - Jong-Jer Lee
humanIHC

Bioengineered

A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma.

Authors - Huimin Shen
humanWB

Autophagy

Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate

Authors - Mengting Ou