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ATP6V1G2 Polyclonal antibody

ATP6V1G2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25316-1-AP

Print datasheet

Synonyms

ATP6G, ATP6G2, ATP6V1G2, NG38, V ATPase 13 kDa subunit 2, V ATPase subunit G 2, VMA10

验证数据展示

  • WB analysis of rat brain using 25316-1-AP

    WB analysis of rat brain using 25316-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25316-1-AP (ATP6V1G2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25316-1-AP

    IHC staining of mouse brain using 25316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25316-1-AP (ATP6V1G2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25316-1-AP

    IHC staining of mouse brain using 25316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25316-1-AP (ATP6V1G2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

25316-1-AP targets ATP6V1G2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ATP6V1G2 fusion protein Ag18007 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2
Synonyms ATP6G, ATP6G2, ATP6V1G2, NG38, V ATPase 13 kDa subunit 2, V ATPase subunit G 2, VMA10
Calculated Molecular Weight 118 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC119726
Gene Symbol ATP6V1G2
Gene ID (NCBI) 534
RRIDAB_2880027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95670
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATP6V1G2 antibody 25316-1-APDownload protocol
IHC protocol for ATP6V1G2 antibody 25316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Microbiol

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Authors - Tomas Doyle
View Article
humanWB

EBioMedicine

A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.

Authors - Irene Bertolini
View Article
humanWB

EBioMedicine

Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo.

Authors - Andrea Terrasi
View Article