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验证数据展示

  • WB analysis of rat brain using 25316-1-AP

    WB analysis of rat brain using 25316-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25316-1-AP (ATP6V1G2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25316-1-AP

    IHC staining of mouse brain using 25316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25316-1-AP (ATP6V1G2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25316-1-AP

    IHC staining of mouse brain using 25316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25316-1-AP (ATP6V1G2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP6V1G2 Polyclonal antibody

ATP6V1G2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25316-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ATP6G, ATP6G2, ATP6V1G2, NG38, V ATPase 13 kDa subunit 2, V ATPase subunit G 2, VMA10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

25316-1-AP targets ATP6V1G2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ATP6V1G2 fusion protein Ag18007 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2
别名 ATP6G, ATP6G2, ATP6V1G2, NG38, V ATPase 13 kDa subunit 2, V ATPase subunit G 2, VMA10
计算分子量 118 aa, 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC119726
基因名称 ATP6V1G2
Gene ID (NCBI) 534
RRIDAB_2880027
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95670
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATP6V1G2 antibody 25316-1-APDownload protocol
IHC protocol for ATP6V1G2 antibody 25316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Microbiol

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Authors - Tomas Doyle
View Article
humanWB

EBioMedicine

A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.

Authors - Irene Bertolini
View Article
humanWB

EBioMedicine

Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo.

Authors - Andrea Terrasi
View Article