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ATP6V1G3 Polyclonal antibody

ATP6V1G3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19523-1-AP

Print datasheet

Synonyms

ATP6G3, ATP6V1G3, V ATPase 13 kDa subunit 3, V ATPase subunit G 3, Vma10

验证数据展示

  • WB analysis of HEK-293 using 19523-1-AP

    WB analysis of HEK-293 using 19523-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 19523-1-AP

    IHC staining of human kidney using 19523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 19523-1-AP

    IHC staining of human kidney using 19523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse kidney using 19523-1-AP

    IP experiment of mouse kidney using 19523-1-AP

    IP result of anti-ATP6V1G3 (IP:19523-1-AP, 4ug; Detection:19523-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19523-1-AP targets ATP6V1G3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
Synonyms ATP6G3, ATP6V1G3, V ATPase 13 kDa subunit 3, V ATPase subunit G 3, Vma10
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberNM_133326
Gene Symbol ATP6V1G3
Gene ID (NCBI) 127124
RRIDAB_10642838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LB4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP6V1G3,also named as ATP6G3, belongs to the V-ATPase G subunit family. ATP6V1G3 is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

实验方案

Product Specific Protocols
WB protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
IHC protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
IP protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols