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ATP6V1H Monoclonal antibody, PBS Only

ATP6V1H Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G7B5

Cat no : 68425-1-PBS

Print datasheet

Synonyms

ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13

验证数据展示

  • WB analysis using 68425-1-Ig (same clone as 68425-1-PBS)

    WB analysis using 68425-1-Ig (same clone as 68425-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68425-1-Ig (ATP6V1H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68425-1-PBS in a different storage buffer formulation.

  • WB analysis using 68425-1-Ig (same clone as 68425-1-PBS)

    WB analysis using 68425-1-Ig (same clone as 68425-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68425-1-Ig (ATP6V1H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68425-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68425-1-Ig (same clone as 68425-1-PBS)

    IF Staining of HepG2 using 68425-1-Ig (same clone as 68425-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP6V1H antibody (68425-1-Ig, Clone: 3G7B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68425-1-PBS in a different storage buffer formulation.

产品信息

68425-1-PBS targets ATP6V1H in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen ATP6V1H fusion protein Ag10130 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
Synonyms ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13
Calculated Molecular Weight 483 aa, 56 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC025275
Gene Symbol ATP6V1H
Gene ID (NCBI) 51606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UI12
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.