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Host
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Species Reactivity
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 68425-1-Ig (same clone as 68425-1-PBS)

    WB analysis using 68425-1-Ig (same clone as 68425-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68425-1-Ig (ATP6V1H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68425-1-PBS in a different storage buffer formulation.

  • WB analysis using 68425-1-Ig (same clone as 68425-1-PBS)

    WB analysis using 68425-1-Ig (same clone as 68425-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68425-1-Ig (ATP6V1H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68425-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68425-1-Ig (same clone as 68425-1-PBS)

    IF Staining of HepG2 using 68425-1-Ig (same clone as 68425-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP6V1H antibody (68425-1-Ig, Clone: 3G7B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68425-1-PBS in a different storage buffer formulation.

ATP6V1H Monoclonal antibody, PBS Only

ATP6V1H Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68425-1-PBS

产品说明书

CloneNo. 3G7B5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, rabbit, pig

应用

WB, IF/ICC, Indirect ELISA

别名

ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68425-1-PBS targets ATP6V1H in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit, pig
免疫原 ATP6V1H fusion protein Ag10130 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
别名 ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13
计算分子量 483 aa, 56 kDa
观测分子量 50 kDa
GenBank蛋白编号BC025275
基因名称 ATP6V1H
Gene ID (NCBI) 51606
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UI12
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.