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ATP8A2 Polyclonal antibody

ATP8A2 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 31697-1-AP

Print datasheet

Synonyms

ML 1, ATPIB

验证数据展示

  • IP experiment of mouse testis using 31697-1-AP

    IP experiment of mouse testis using 31697-1-AP

    IP result of anti-ATP8A2 (IP:31697-1-AP, 4ug; Detection:31697-1-AP 1:600) with mouse testis tissue lysate 1120 ug.

  • IHC staining of mouse brain using 31697-1-AP

    IHC staining of mouse brain using 31697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IP detected inmouse testis tissue
Positive IHC detected inmouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31697-1-AP targets ATP8A2 in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATP8A2 fusion protein Ag36276 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, aminophospholipid transporter-like, class I, type 8A, member 2
Synonyms ML 1, ATPIB
Calculated Molecular Weight133kDa
Observed Molecular Weight129 kDa
GenBank Accession NumberNM_001313741
Gene Symbol ATP8A2
Gene ID (NCBI) 51761
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NTI2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP8A2 is also known as ATPIB. ATP8A2 is a member of the P4‐ATPase subfamily of P‐type ATPases that actively flips phosphatidylserine (PS) and phosphatidylethanolamine (PE) across membranes to generate and maintain transmembrane phospholipid asymmetry, a property important in such cellular processes as vesicle trafficking, apoptosis, cytokinesis, neuron survival, blood coagulation, and phagocytosis. ATP8A2 consists of an N‐domain involved in binding ATP, a P‐domain that undergoes phosphorylation, an A‐domain that functions in the dephosphorylation of the phosphorylated intermediate, and an M‐domain consisting of 10 transmembrane segments that comprise the pathway for translocation of the lipid across the membrane. ATP8A2 is highly expressed in the brain, spinal cord, testis, and retina (PMID: 33565221, PMID: 33766557, PMID: 37635783).

实验方案

Product Specific Protocols
IHC protocol for ATP8A2 antibody 31697-1-APDownload protocol
IP protocol for ATP8A2 antibody 31697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols