• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATP9A Polyclonal antibody

ATP9A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19504-1-AP

Print datasheet

Synonyms

Probable phospholipid-transporting ATPase IIA, KIAA0611, EC:7.6.2.1, ATPIIA

验证数据展示

  • WB analysis of mouse spleen using 19504-1-AP

    WB analysis of mouse spleen using 19504-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19504-1-AP (ATP9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 19504-1-AP

    IHC staining of human testis using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 19504-1-AP

    IHC staining of human testis using 19504-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 19504-1-AP

    IF Staining of HeLa using 19504-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19504-1-AP (ATP9A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman testis tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19504-1-AP targets ATP9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, class II, type 9A
Synonyms Probable phospholipid-transporting ATPase IIA, KIAA0611, EC:7.6.2.1, ATPIIA
Calculated Molecular Weight 119 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberNM_006045
Gene Symbol ATP9A
Gene ID (NCBI) 10079
RRIDAB_10640806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75110
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP9A, also named as ATPIIA and KIAA0611, belongs to the cation transport ATPase (P-type) family and Type IV subfamily. ATP9A catalyzes the reaction: ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).

实验方案

Product Specific Protocols
WB protocol for ATP9A antibody 19504-1-APDownload protocol
IHC protocol for ATP9A antibody 19504-1-APDownload protocol
IF protocol for ATP9A antibody 19504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols