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ATPAF1 Polyclonal antibody

ATPAF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15797-1-AP

Print datasheet

Synonyms

ATP11p, ATP11 homolog, ATP11

验证数据展示

  • WB analysis of L02 using 15797-1-AP

    WB analysis of L02 using 15797-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15797-1-AP (ATPAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 15797-1-AP

    IHC staining of human ovary cancer using 15797-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15797-1-AP (ATPAF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 15797-1-AP

    IHC staining of human stomach cancer using 15797-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15797-1-AP (ATPAF1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inhuman ovary cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15797-1-AP targets ATPAF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATPAF1 fusion protein Ag8506 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP synthase mitochondrial F1 complex assembly factor 1
Synonyms ATP11p, ATP11 homolog, ATP11
Calculated Molecular Weight 328 aa, 36 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC008498
Gene Symbol ATPAF1
Gene ID (NCBI) 64756
RRIDAB_2243354
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5TC12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATPAF1 antibody 15797-1-APDownload protocol
IHC protocol for ATPAF1 antibody 15797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols