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ATPAF2 Polyclonal antibody

ATPAF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11598-1-AP

Print datasheet

Synonyms

ATP12, ATP12 homolog, ATP12p, ATPAF2, LP3663

验证数据展示

  • WB analysis of HEK-293 using 11598-1-AP

    WB analysis of HEK-293 using 11598-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11598-1-AP (ATPAF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11598-1-AP

    IHC staining of human colon cancer using 11598-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11598-1-AP (ATPAF2 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11598-1-AP targets ATPAF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen ATPAF2 fusion protein Ag2164 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP synthase mitochondrial F1 complex assembly factor 2
Synonyms ATP12, ATP12 homolog, ATP12p, ATPAF2, LP3663
Calculated Molecular Weight 289 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC032126
Gene Symbol ATPAF2
Gene ID (NCBI) 91647
RRIDAB_2063032
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATPAF2 antibody 11598-1-APDownload protocol
IHC protocol for ATPAF2 antibody 11598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Neurobiol Aging

The perimenopausal aging transition in the female rat brain: decline in bioenergetic systems and synaptic plasticity.

Authors - Fei Yin
View Article