• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATPAF2 Polyclonal antibody

ATPAF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30334-1-AP

Print datasheet

Synonyms

ATP12, LP3663, ATP12p, ATP12 homolog

验证数据展示

  • WB analysis using 30334-1-AP

    WB analysis using 30334-1-AP

    Mouse liver tissues (total crude protein, mitochondrial protein) were subjected to SDS PAGE followed by western blot with 30334-1-AP (ATPAF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 30334-1-AP

    IHC staining of human stomach cancer using 30334-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30334-1-AP (ATPAF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30334-1-AP targets ATPAF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATPAF2 fusion protein Ag33299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP synthase mitochondrial F1 complex assembly factor 2
Synonyms ATP12, LP3663, ATP12p, ATP12 homolog
Calculated Molecular Weight 289 aa, 33 kDa
Observed Molecular Weight~30 kDa
GenBank Accession NumberBC032126
Gene Symbol ATPAF2
Gene ID (NCBI) 91647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) also name as ATP12 and ATP12p, is an essential house keeping protein. ATPAF2 is an assembly factor for the F1 component of mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. The calculated MW is 33 kDa, 30334-1-AP can detect bands around 30 kDa and 50 kDa, the larger band may correspond to dimer or complex form. (PMID: 1826907, 11410595)

实验方案

Product Specific Protocols
WB protocol for ATPAF2 antibody 30334-1-APDownload protocol
IHC protocol for ATPAF2 antibody 30334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols