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验证数据展示

  • WB analysis using 30334-1-AP

    WB analysis using 30334-1-AP

    Mouse liver tissues (total crude protein, mitochondrial protein) were subjected to SDS PAGE followed by western blot with 30334-1-AP (ATPAF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 30334-1-AP

    IHC staining of human stomach cancer using 30334-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30334-1-AP (ATPAF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATPAF2 Polyclonal antibody

ATPAF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30334-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ATP12, LP3663, ATP12p, ATP12 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30334-1-AP targets ATPAF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ATPAF2 fusion protein Ag33299 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP synthase mitochondrial F1 complex assembly factor 2
别名 ATP12, LP3663, ATP12p, ATP12 homolog
计算分子量 289 aa, 33 kDa
观测分子量~30 kDa
GenBank蛋白编号BC032126
基因名称 ATPAF2
Gene ID (NCBI) 91647
RRIDAB_3086297
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N5M1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) also name as ATP12 and ATP12p, is an essential house keeping protein. ATPAF2 is an assembly factor for the F1 component of mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. The calculated MW is 33 kDa, 30334-1-AP can detect bands around 30 kDa and 50 kDa, the larger band may correspond to dimer or complex form. (PMID: 1826907, 11410595)

实验方案

Product Specific Protocols
WB protocol for ATPAF2 antibody 30334-1-APDownload protocol
IHC protocol for ATPAF2 antibody 30334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols