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验证数据展示

  • WB analysis using 31537-1-AP

    WB analysis using 31537-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 31537-1-AP (ATPBD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 31537-1-AP

    IHC staining of mouse kidney using 31537-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31537-1-AP (ATPBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATPBD3 antibody included
  • IF Staining of PC-3 using 31537-1-AP

    IF Staining of PC-3 using 31537-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ATPBD3 antibody (31537-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

ATPBD3 Polyclonal antibody

ATPBD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31537-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

NCS6, CTU1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy® ATPBD3 Ready-To-Use IHC Kit. ATPBD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31537-1-AP targets ATPBD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35037

Product name: Recombinant human ATPBD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-200 aa of NM_145232

Sequence: PGAVVAVGASGGKDSTVLAHVLRALAPRLGISLQLVAVDEGIGGYRDAALAAVRRQAARWELPLTVVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARRVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGGLGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP binding domain 3
别名 NCS6, CTU1
计算分子量36 kDa
观测分子量 30-36 kDa
GenBank蛋白编号NM_145232
基因名称 ATPBD3
Gene ID (NCBI) 90353
RRIDAB_3670027
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ7Z7A3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATPBD3, also known as CTU1, NCS6, is a subunit of a highly conserved protein complex involved in the thiolation of wobble U34(PMID: 18391219). ATPBD3 directly binds tRNAs and probably acts by catalyzing the adenylation of tRNAs, an intermediate required for 2-thiolation.

实验方案

Product Specific Protocols
IF protocol for ATPBD3 antibody 31537-1-APDownload protocol
IHC protocol for ATPBD3 antibody 31537-1-APDownload protocol
WB protocol for ATPBD3 antibody 31537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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