• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATPBD3 Polyclonal antibody

ATPBD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31537-1-AP

Print datasheet

Synonyms

NCS6, CTU1

验证数据展示

  • WB analysis using 31537-1-AP

    WB analysis using 31537-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 31537-1-AP (ATPBD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 31537-1-AP

    IHC staining of mouse kidney using 31537-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31537-1-AP (ATPBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 31537-1-AP

    IF Staining of PC-3 using 31537-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ATPBD3 antibody (31537-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31537-1-AP targets ATPBD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ATPBD3 fusion protein Ag35037 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP binding domain 3
Synonyms NCS6, CTU1
Calculated Molecular Weight36 kDa
Observed Molecular Weight 30-36 kDa
GenBank Accession NumberNM_145232
Gene Symbol ATPBD3
Gene ID (NCBI) 90353
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ7Z7A3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATPBD3, also known as CTU1, NCS6, is a subunit of a highly conserved protein complex involved in the thiolation of wobble U34(PMID: 18391219). ATPBD3 directly binds tRNAs and probably acts by catalyzing the adenylation of tRNAs, an intermediate required for 2-thiolation.

实验方案

Product Specific Protocols
WB protocol for ATPBD3 antibody 31537-1-APDownload protocol
IHC protocol for ATPBD3 antibody 31537-1-APDownload protocol
IF protocol for ATPBD3 antibody 31537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols