• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATPBD4 Monoclonal antibody, PBS Only

ATPBD4 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B12E1

Cat no : 68397-1-PBS

Print datasheet

Synonyms

ATP binding domain 4, ATPBD4, DPH6

验证数据展示

  • WB analysis using 68397-1-Ig (same clone as 68397-1-PBS)

    WB analysis using 68397-1-Ig (same clone as 68397-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68397-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 68397-1-Ig (same clone as 68397-1-PBS)

    WB analysis of K-562 using 68397-1-Ig (same clone as 68397-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68397-1-PBS in a different storage buffer formulation.

产品信息

68397-1-PBS targets ATPBD4 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen ATPBD4 fusion protein Ag33218 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP binding domain 4
Synonyms ATP binding domain 4, ATPBD4, DPH6
Calculated Molecular Weight 267 aa, 30 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC008485
Gene Symbol ATPBD4
Gene ID (NCBI) 89978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7L8W6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Diphthamide is a highly modified histidine in the transcription factor EEF2 that is conserved from yeast to human. ATPBD4, also known as DPH6, belongs to the Diphthine--ammonia ligase family. DPH6 amidase may catalyze the last step of diphthamide biosynthesis using ammonium and ATP to play a role in catalyzing the final step of diphthamide biosynthesis, amidation of diphthine to diphthamide (PubMed:23169644).