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ATRAID Polyclonal antibody

ATRAID Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25548-1-AP

Print datasheet

Synonyms

Apoptosis related protein 3, APR 3, APR 3, APR3, C2orf28, HSPC013, p18, PRO240, UNQ214/PRO240

验证数据展示

  • WB analysis of mouse brain using 25548-1-AP

    WB analysis of mouse brain using 25548-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25548-1-AP (ATRAID antibody at dilution of 1:300 incubated at 4 degree celsius over night.

经过测试的应用

Positive WB detected inmouse brain tissue, HEK-293 cells, mouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25548-1-AP is ATRAID Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATRAID fusion protein Ag22168 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 2 open reading frame 28
Synonyms Apoptosis related protein 3, APR 3, APR 3, APR3, C2orf28, HSPC013, p18, PRO240, UNQ214/PRO240
Calculated Molecular Weight 229 aa, 25 kDa
Observed Molecular Weight 28-35 kDa
GenBank Accession NumberBC035850
Gene Symbol ATRAID
Gene ID (NCBI) 51374
RRIDAB_2880128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UW56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATRAID, also named as APR3, p18, C2orf28, promotes osteoblast cell differentiation and terminal mineralization. It plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway. This antibody recognizes all the three isoforms (20-24 kDa, 16-19 kDa and 28-30 kDa).

实验方案

Product Specific Protocols
WB protocol for ATRAID antibody 25548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols