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验证数据展示

  • WB analysis using 11327-1-AP

    WB analysis using 11327-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 11327-1-AP

    WB analysis of HeLa using 11327-1-AP

    WB result of ATRIP antibody (11327-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATRIP transfected HeLa cells.

  • IP experiment of HeLa using 11327-1-AP

    IP experiment of HeLa using 11327-1-AP

    IP result of anti-ATRIP (IP:11327-1-AP, 4ug; Detection:11327-1-AP 1:5000) with HeLa cells lysate 1200 ug.

  • Featured Product
  • KD/KO验证

ATRIP Polyclonal antibody

ATRIP Polyclonal Antibody for WB, IP, ELISA
Cat No. 11327-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA and More (1)

别名

AGS1, ATR interacting protein, ATRIP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, PC-3 cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11327-1-AP targets ATRIP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 ATRIP fusion protein Ag1869 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATR interacting protein
别名 AGS1, ATR interacting protein, ATRIP
计算分子量 791 aa, 86 kDa
观测分子量 86-90 kDa
GenBank蛋白编号BC014153
基因名称 ATRIP
Gene ID (NCBI) 84126
RRIDAB_10837229
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXE1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATRIP (ATR Interacting Protein) and ATR signalling complex is a central mediator of the replicative stress response (RSR),and mutations in both genes have been reported in Seckel syndrome.

实验方案

Product Specific Protocols
WB protocol for ATRIP antibody 11327-1-APDownload protocol
IP protocol for ATRIP antibody 11327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Biomaterials

A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction.

Authors - Jin Qiu
  • KD Validated
View Article
humanWB

Front Pharmacol

Huangkui Capsule Ameliorates Renal Fibrosis in a Unilateral Ureteral Obstruction Mouse Model Through TRPC6 Dependent Signaling Pathways.

Authors - Li-Fei Gu
View Article
mouseWB

Biochem J

Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance

Authors - Jill E Hunter
View Article
mouseWB

Biochem J

Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway

Authors - Jill E Hunter
View Article
humanWB

Cell Insight

ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance

Authors - Rong Luo
View Article