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ATRX Polyclonal antibody, PBS Only

ATRX Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20495-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 20495-1-AP (same clone as 20495-1-PBS)

    WB analysis using 20495-1-AP (same clone as 20495-1-PBS)

    A549 cells were subjected to Tris-Acetate gel system followed by western blot with 20495-1-AP (ATRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

    IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

    IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 20495-1-AP (same clone as 20495-1-PBS)

    IF Staining of A549 using 20495-1-AP (same clone as 20495-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATRX antibody (20495-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

产品信息

20495-1-PBS targets ATRX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATRX fusion protein Ag13984 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)
Synonyms
Calculated Molecular Weight 2492 aa, 283 kDa
Observed Molecular Weight280-300 kDa
GenBank Accession NumberBC002521
Gene Symbol ATRX
Gene ID (NCBI) 546
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46100
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The α-thalassemia mental retardation X-linked protein (ATRX) is a member of the Switch 2, sucrose non-fermenting 2 (SWI2/SNF2) family of helicases/ATPases that exhibits chromatin remodeling activity. ATRX contains an atypical plant homeodomain (PHD) finger domain that recognizes a combinatorial modification pattern on histone H3 tails. ATRX mutations in cancer are most commonly found in pediatric and adolescent malignancies including glioma, neuroblastoma, adrenocortical carcinoma and osteosarcoma(PMID: 32015155).