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Host
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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis using 20495-1-AP (same clone as 20495-1-PBS)

    WB analysis using 20495-1-AP (same clone as 20495-1-PBS)

    A549 cells were subjected to Tris-Acetate gel system followed by western blot with 20495-1-AP (ATRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

    IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

    IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 20495-1-AP (same clone as 20495-1-PBS)

    IF Staining of A549 using 20495-1-AP (same clone as 20495-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATRX antibody (20495-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

ATRX Polyclonal antibody, PBS Only

ATRX Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20495-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

20495-1-PBS targets ATRX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATRX fusion protein Ag13984 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)
别名
计算分子量 2492 aa, 283 kDa
观测分子量280-300 kDa
GenBank蛋白编号BC002521
基因名称 ATRX
Gene ID (NCBI) 546
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46100
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The α-thalassemia mental retardation X-linked protein (ATRX) is a member of the Switch 2, sucrose non-fermenting 2 (SWI2/SNF2) family of helicases/ATPases that exhibits chromatin remodeling activity. ATRX contains an atypical plant homeodomain (PHD) finger domain that recognizes a combinatorial modification pattern on histone H3 tails. ATRX mutations in cancer are most commonly found in pediatric and adolescent malignancies including glioma, neuroblastoma, adrenocortical carcinoma and osteosarcoma(PMID: 32015155).