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验证数据展示

  • WB analysis of human brain using 15693-1-AP

    WB analysis of human brain using 15693-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15693-1-AP

    IHC staining of human ovary tumor using 15693-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human ovary tumor using 15693-1-AP

    IHC staining of human ovary tumor using 15693-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 15693-1-AP

    IF Staining of HeLa using 15693-1-AP

    Immunofluorescent analysis of Hela cells, using ATXN10 antibody 15693-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of A431 using 15693-1-AP

    IP experiment of A431 using 15693-1-AP

    IP result of anti-ATXN10 (IP:15693-1-AP, 4ug; Detection:15693-1-AP 1:1000) with A431 cells lysate 1200ug.

ATXN10 Polyclonal antibody

ATXN10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15693-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

SCA10, E46L, Ataxin-10, ataxin 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, human kidney tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15693-1-AP targets ATXN10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 ATXN10 fusion protein Ag8259 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ataxin 10
别名 SCA10, E46L, Ataxin-10, ataxin 10
计算分子量 53 kDa
观测分子量 50 kDa
GenBank蛋白编号BC007508
基因名称 ATXN10
Gene ID (NCBI) 25814
RRIDAB_2061167
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBB4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

实验方案

Product Specific Protocols
WB protocol for ATXN10 antibody 15693-1-APDownload protocol
IHC protocol for ATXN10 antibody 15693-1-APDownload protocol
IF protocol for ATXN10 antibody 15693-1-APDownload protocol
IP protocol for ATXN10 antibody 15693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.

Authors - Sang Liyun L
View Article
humanWB,IF

bioRxiv

Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties

Authors - Harihar M Mohan
View Article
humanWB

J Neurochem

Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.

Authors - Robert J Andrew
View Article