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ATXN10 Polyclonal antibody

ATXN10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15693-1-AP

Print datasheet

Synonyms

SCA10, E46L, Ataxin-10, ataxin 10

验证数据展示

  • WB analysis of human brain using 15693-1-AP

    WB analysis of human brain using 15693-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15693-1-AP

    IHC staining of human ovary tumor using 15693-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human ovary tumor using 15693-1-AP

    IHC staining of human ovary tumor using 15693-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 15693-1-AP

    IF Staining of HeLa using 15693-1-AP

    Immunofluorescent analysis of Hela cells, using ATXN10 antibody 15693-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of A431 using 15693-1-AP

    IP experiment of A431 using 15693-1-AP

    IP result of anti-ATXN10 (IP:15693-1-AP, 4ug; Detection:15693-1-AP 1:1000) with A431 cells lysate 1200ug.

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, human kidney tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15693-1-AP targets ATXN10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ATXN10 fusion protein Ag8259 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ataxin 10
Synonyms SCA10, E46L, Ataxin-10, ataxin 10
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC007508
Gene Symbol ATXN10
Gene ID (NCBI) 25814
RRIDAB_2061167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBB4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

实验方案

Product Specific Protocols
WB protocol for ATXN10 antibody 15693-1-APDownload protocol
IHC protocol for ATXN10 antibody 15693-1-APDownload protocol
IF protocol for ATXN10 antibody 15693-1-APDownload protocol
IP protocol for ATXN10 antibody 15693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.

Authors - Sang Liyun L
View Article
humanWB

J Neurochem

Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.

Authors - Robert J Andrew
View Article