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验证数据展示

  • WB analysis using 68455-1-Ig

    WB analysis using 68455-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68455-1-Ig (ATXN10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

ATXN10 Monoclonal antibody

ATXN10 Monoclonal Antibody for WB, ELISA
Cat No. 68455-1-Ig

产品说明书

CloneNo. 1B4E4

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, ELISA

别名

ataxin 10, ATXN10, Brain protein E46 homolog, E46L, FLJ37990, SCA10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68455-1-Ig targets ATXN10 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 ATXN10 fusion protein Ag8254 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ataxin 10
别名 ataxin 10, ATXN10, Brain protein E46 homolog, E46L, FLJ37990, SCA10
计算分子量 53 kDa
观测分子量45 kDa
GenBank蛋白编号BC007508
基因名称 ATXN10
Gene ID (NCBI) 25814
RRIDAB_3085167
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBB4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

实验方案

Product Specific Protocols
WB protocol for ATXN10 antibody 68455-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols