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验证数据展示

  • WB analysis of human testis using 23166-1-AP

    WB analysis of human testis using 23166-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 23166-1-AP (ATXN3L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23166-1-AP

    IHC staining of human testis using 23166-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23166-1-AP (ATXN3L Antibody) at dilution of 1:200 (under 40x lens).

ATXN3L Polyclonal antibody

ATXN3L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23166-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ataxin 3 like, ATX3L, ATXN3L, MJDL, Putative ataxin 3 like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23166-1-AP targets ATXN3L in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ATXN3L fusion protein Ag19611 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ataxin 3-like
别名 ataxin 3 like, ATX3L, ATXN3L, MJDL, Putative ataxin 3 like protein
计算分子量 355 aa, 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC137187
基因名称 ATXN3L
Gene ID (NCBI) 92552
RRIDAB_2879221
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3M9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATXN3L, also named as Ataxin-3-like protein, is a 355 amino acid protein, which contains 1 Josephin domain and 2 UIM (ubiquitin-interacting motif) domains. ATXN3L localizes in the nucleus and as a deubiquitinating enzyme that cleaves both 'Lys-48'-linked and 'Lys-63'-linked poly-ubiquitin chains.

实验方案

Product Specific Protocols
WB protocol for ATXN3L antibody 23166-1-APDownload protocol
IHC protocol for ATXN3L antibody 23166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols