• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

AURKA Monoclonal antibody, PBS Only

AURKA Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F4B10

Cat No : 66757-1-PBS

Print datasheet

Synonyms

AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

验证数据展示

  • IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AURKA antibody included
  • IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AURKA antibody included
  • WB analysis of human testis using 66757-1-Ig (same clone as 66757-1-PBS)

    WB analysis of human testis using 66757-1-Ig (same clone as 66757-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

    IF Staining of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (66757-1-Ig, Clone: 1F4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • WB analysis using 66757-1-Ig (same clone as 66757-1-PBS)

    WB analysis using 66757-1-Ig (same clone as 66757-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AURKA Ready-To-Use IHC Kit. AURKA primary antibody included.

产品信息

66757-1-PBS targets AURKA in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen AURKA fusion protein Ag20096 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name aurora kinase A
Synonyms AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46-52 kDa
GenBank Accession NumberBC002499
Gene Symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14965
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).