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AURKA Recombinant antibody, PBS Only

AURKA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230087H3

Cat no : 82906-1-PBS

Print datasheet

Synonyms

Aurora A, AURK, AURA, ARK-1, AIRK1

验证数据展示

  • WB analysis using 82906-1-RR (same clone as 82906-1-PBS)

    WB analysis using 82906-1-RR (same clone as 82906-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82906-1-RR (AURKA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

    IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AURKA antibody (82906-1-RR, Clone: 230087H3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

    IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AURKA antibody (82906-1-RR, Clone: 230087H3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82906-1-RR

    Affinity and Kinetic Characterization of 82906-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82906-1-RR against Human AURKA were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy AURKA Ready-To-Use IHC Kit. AURKA primary antibody included.

产品信息

82906-1-PBS targets AURKA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen AURKA fusion protein Ag0362 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name aurora kinase A
Synonyms Aurora A, AURK, AURA, ARK-1, AIRK1
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002499
Gene Symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14965
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).