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CoraLite®594-conjugated AURKA Monoclonal antibody

AURKA Monoclonal Antibody for IF
Cat No. CL594-66757

产品说明书

CloneNo. 1F4B10

宿主/亚型

Mouse / IgG3

种属反应性

Human, Mouse, Rat

应用

IF

AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AURKA Ready-To-Use IHC Kit. AURKA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66757-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66757 targets AURKA in IF applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF Application Description
经测试反应性 Human, Mouse, Rat
免疫原 AURKA fusion protein Ag20096 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 aurora kinase A
别名 AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7
计算分子量 46 kDa
观测分子量 46-52 kDa
GenBank蛋白编号BC002499
基因名称 AURKA
Gene ID (NCBI) 6790
RRIDAB_2883624
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14965
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

实验方案

Product Specific Protocols
IF protocol for CL594 AURKA antibody CL594-66757Download protocol
Standard Protocols
Click here to view our Standard Protocols
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