验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, NCI-H1299 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11548-1-AP targets Antizyme inhibitor 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Antizyme inhibitor 1 fusion protein Ag2082 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | antizyme inhibitor 1 |
| 别名 | AZIN1, OAZIN, OAZI, AZIN 1, AZI1 |
| 计算分子量 | 49.5 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC019279 |
| 基因名称 | AZIN1 |
| Gene ID (NCBI) | 51582 |
| RRID | AB_2290243 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14977 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AZIN1(Antizyme inhibitor 1) is also named as OAZI, OAZIN and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine in the first and apparently rate-limiting step in polyamine biosynthesis, and playing a role in the regulation of polyamine synthesis (negative regulator of cellular polyamines).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Antizyme inhibitor 1 antibody 11548-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell The Genomic Landscape and Clinical Relevance of A-to-I RNA Editing in Human Cancers. | ||
J Clin Invest Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma. | ||
Mol Ther Targeting RNA Editing of Antizyme Inhibitor 1: a Potential Oligonucleotide-Based Antisense Therapy for Cancer. | ||
Kidney Int The microRNA miR-433 promotes renal fibrosis by amplifying the TGF-β/Smad3-Azin1 pathway. | ||
FASEB J The rational discovery of multipurpose inhibitors of the ornithine decarboxylase. |