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AbFlex BRD4 antibody (rAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP, ChIP-Seq

Cat No : 91301,91302 91301

Synonyms

BRD4, Bromodomain-containing protein 4, HUNK1, brd 4, brd-4, brd, bromodomain, bromo domain, MCAP, Recombinant Antibody

验证数据展示

  • AbFlex BRD4 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of HCT-15 cells (human colorectal adenocarcinoma line chromatin) and 4 ug of BRD4 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14.5 million sequence tags were mapped to identify BRD4 binding sites on chromosome 19.

    AbFlex BRD4 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of HCT-15 cells (human colorectal adenocarcinoma line chromatin) and 4 ug of BRD4 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14.5 million sequence tags were mapped to identify BRD4 binding sites on chromosome 19.

产品信息

Tested Applications ChIP, ChIP-Seq

Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen This antibody was raised against a recombinant protein comprising amino acids 149-284 of human BRD4.
Full Name AbFlex BRD4 antibody (rAb)
Synonyms BRD4, Bromodomain-containing protein 4, HUNK1, brd 4, brd-4, brd, bromodomain, bromo domain, MCAP, Recombinant Antibody
Molecular weight 180 kDa
GenBank accession numberNP_490597
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex BRD4 antibody was expressed in 293 cells and contains rabbit immunoglobulin heavy and light chains. BRD4 (Bromodomain-containing protein 4) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female Sterile Homeotic protein gene in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD4 has been identified recently as a therapeutic target in many cancers, including acute myeloid leukemia, multiple myeloma, Burkitt’s lymphoma, NUT midline carcinoma, colon cancer, and breast cancer. BRD4 regulates the transcription of oncogenes, HIV, and human papilloma virus (HPV). It has been shown to bind and phosphorylate RNA pol II, which implicates its involvement in the regulation of eukaryotic transcription. It shows binding specificity for acetylated H3K9, H3K9/ K14, H4K5, H4K8, H4K12, H4K5/K8, H4K5/K12, H4K8/K12, H4K12/K16, H4K12/K16/K20 and H4K5/K8/K12/K16, as well as acetylated RelA-K310.